- Use the Leica stereo scope equipped with the reflection stage
- Place the substrate in the horizontal position in the field of view, ensuring that the substrate surfaces is horizontal and parallel wit the optical path
- Use the micromanipulator and a syringe to place a small drop of fresh nanopure water on the surface
- Collect and image using the software “micromanager”
- Process the image using the contact angle procedure in imagej
(note: this is a cursory overview, needs to be tested and elaborated by an experience user then a novice user)